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itc 200 instrument  (Malvern Panalytical)


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    Structured Review

    Malvern Panalytical itc 200 instrument
    Itc 200 Instrument, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/itc+200/pmc13061415-276-19-21
    Average 86 stars, based on 1 article reviews
    itc 200 instrument - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Purification:

    Article Title: SHANK3 depletion leads to ERK signalling overdose and cell death in KRAS-mutant cancers
    Article Snippet: The dissociation constant was then calculated using a single-site binding model to fit the curve using GraphPad Prism version 8.4.2 for Windows (GraphPad Software Inc.). .. For ITC measurements, the buffer of purified recombinant proteins (SPN-ARR fragment of SHANK3, GMPPCP-or GDP-loaded His6-KRASQ61H and RBD domain of human BRAF) was exchanged for ITC buffer (20 mM Tris pH 7.5, 500 mM NaCl, 0.5 mM TCEP (tris-carboxyethyl-phosphine) and 5 mM MgCl 2 ) and ITC experiments were performed using an ITC-200 (Microcal). ..

    Article Title: SHANK3 depletion leads to ERK signalling overdose and cell death in KRAS-mutant cancers.
    Article Snippet: The dissociation constant was then calculated using a single-site binding model to fit the curve using GraphPad Prism version 8.4.2 for Windows (GraphPad Software Inc.). .. For ITC measurements, the buffer of purified recombinant proteins (SPN-ARR fragment of SHANK3, GMPPCP-or GDP-loaded His6KRASQ61H and RBD domain of human BRAF) was exchanged for ITC buffer (20mM Tris pH 7.5, 500mM NaCl, 0.5mM TCEP (tris-carboxyethyl-phosphine) and 5mM MgCl2) and ITC experiments were performed using an ITC-200 (Microcal). ..

    Recombinant:

    Article Title: SHANK3 depletion leads to ERK signalling overdose and cell death in KRAS-mutant cancers
    Article Snippet: The dissociation constant was then calculated using a single-site binding model to fit the curve using GraphPad Prism version 8.4.2 for Windows (GraphPad Software Inc.). .. For ITC measurements, the buffer of purified recombinant proteins (SPN-ARR fragment of SHANK3, GMPPCP-or GDP-loaded His6-KRASQ61H and RBD domain of human BRAF) was exchanged for ITC buffer (20 mM Tris pH 7.5, 500 mM NaCl, 0.5 mM TCEP (tris-carboxyethyl-phosphine) and 5 mM MgCl 2 ) and ITC experiments were performed using an ITC-200 (Microcal). ..

    Article Title: SHANK3 depletion leads to ERK signalling overdose and cell death in KRAS-mutant cancers.
    Article Snippet: The dissociation constant was then calculated using a single-site binding model to fit the curve using GraphPad Prism version 8.4.2 for Windows (GraphPad Software Inc.). .. For ITC measurements, the buffer of purified recombinant proteins (SPN-ARR fragment of SHANK3, GMPPCP-or GDP-loaded His6KRASQ61H and RBD domain of human BRAF) was exchanged for ITC buffer (20mM Tris pH 7.5, 500mM NaCl, 0.5mM TCEP (tris-carboxyethyl-phosphine) and 5mM MgCl2) and ITC experiments were performed using an ITC-200 (Microcal). ..

    Binding Assay:

    Article Title: BMX-001, a clinically relevant radioprotector, can reverse radiation-induced fibrosis when given three weeks after radiation, in part, by restoring methylation
    Article Snippet: Data are presented in GraphPad Prism. .. DNA binding was evaluated using isothermal titration calorimetry (ITC) using the ITC 200 from Malvern (Northampton, MA). ..

    Isothermal Titration Calorimetry:

    Article Title: BMX-001, a clinically relevant radioprotector, can reverse radiation-induced fibrosis when given three weeks after radiation, in part, by restoring methylation
    Article Snippet: Data are presented in GraphPad Prism. .. DNA binding was evaluated using isothermal titration calorimetry (ITC) using the ITC 200 from Malvern (Northampton, MA). ..



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    Malvern Panalytical itc 200 microcalorimeter
    ( A ) Domain architecture of GhWRKY17 and schematic of W-box elements in GhHOX3 and GhMYB109 promoters. ( B ) Isothermal titration calorimetry (ITC) curves for GhWRKY17 WRKY domain (residues 239–304) binding to 12 bp W-box duplexes (HOX3-1, MYB109-1, and MYB109-3). Dissociation constants ( K d , μM) were calculated from heat differentials after buffer control subtraction. Data represent two independent replicates (MicroCal <t>iTC-200).</t>
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    Image Search Results


    ( A ) Domain architecture of GhWRKY17 and schematic of W-box elements in GhHOX3 and GhMYB109 promoters. ( B ) Isothermal titration calorimetry (ITC) curves for GhWRKY17 WRKY domain (residues 239–304) binding to 12 bp W-box duplexes (HOX3-1, MYB109-1, and MYB109-3). Dissociation constants ( K d , μM) were calculated from heat differentials after buffer control subtraction. Data represent two independent replicates (MicroCal iTC-200).

    Journal: Biochemical Journal

    Article Title: Structural basis for sequence-specific DNA recognition by a group IId WRKY transcription factor GhWRKY17 in cotton

    doi: 10.1042/BCJ20250191

    Figure Lengend Snippet: ( A ) Domain architecture of GhWRKY17 and schematic of W-box elements in GhHOX3 and GhMYB109 promoters. ( B ) Isothermal titration calorimetry (ITC) curves for GhWRKY17 WRKY domain (residues 239–304) binding to 12 bp W-box duplexes (HOX3-1, MYB109-1, and MYB109-3). Dissociation constants ( K d , μM) were calculated from heat differentials after buffer control subtraction. Data represent two independent replicates (MicroCal iTC-200).

    Article Snippet: ITC experiments were performed on an iTC-200 microcalorimeter (Malvern Panalytical) at 25°C.

    Techniques: Isothermal Titration Calorimetry, Binding Assay, Control